| POS | Rank | Heat | BIB | ATHLETE | COUNTRY | RESULTS | ms* | 
|---|---|---|---|---|---|---|---|
| 1 | 1 | 3 | 2742 | Karsten WARHOLM |  NOR | 47.09 Q | |
| 2 | 1 | 2 | 2971 | Rai BENJAMIN |  USA | 47.24 Q | |
| 3 | 2 | 3 | 2537 | Roshawn CLARKE |  JAM | 47.34 Q WU20R | |
| 4 | 3 | 3 | 2968 | Trevor BASSITT |  USA | 47.38 q PB | .379 | 
| 5 | 2 | 2 | 2113 | Alison DOS SANTOS |  BRA | 47.38 Q SB | .380 | 
| 6 | 1 | 1 | 2526 | Kyron MCMASTER |  IVB | 47.72 Q | |
| 7 | 2 | 1 | 2261 | Rasmus MÄGI |  EST | 48.30 Q | |
| 8 | 4 | 3 | 2362 | Joshua ABUAKU |  GER | 48.39 q | |
| 9 | 5 | 3 | 2515 | Alessandro SIBILIO |  ITA | 48.43 | |
| 10 | 3 | 1 | 2965 | CJ ALLEN |  USA | 48.44 | |
| 11 | 3 | 2 | 2331 | Ludvy VAILLANT |  FRA | 48.48 | |
| 12 | 4 | 1 | 2546 | Jaheel HYDE |  JAM | 48.49 | |
| 13 | 4 | 2 | 2578 | Kazuki KUROKAWA |  JPN | 48.58 PB | |
| 14 | 6 | 3 | 2929 | Yasmani COPELLO |  TUR | 48.66 SB | |
| 15 | 5 | 2 | 2363 | Emil AGYEKUM |  GER | 48.71 PB | |
| 16 | 7 | 3 | 2310 | Wilfried HAPPIO |  FRA | 48.83 | |
| 17 | 5 | 1 | 2090 | Julien WATRIN |  BEL | 48.94 | |
| 18 | 6 | 1 | 2721 | Ezekiel NATHANIEL |  NGR | 49.22 | |
| 19 | 8 | 3 | 2189 | Gerald DRUMMOND |  CRC | 49.31 | |
| 20 | 6 | 2 | 2623 | Wiseman Were MUKHOBE |  KEN | 49.40 | |
| 21 | 7 | 1 | 2813 | Bassem HEMEIDA |  QAT | 49.50 | |
| 22 | 7 | 2 | 2171 | Zhiyu XIE |  CHN | 49.57 | |
| 23 | 8 | 2 | 2867 | Julien BONVIN |  SUI | 49.75 | |
| 1 | 2504 | Mario LAMBRUGHI |  ITA | DQ | 
ms - Milliseconds - shown when two or more competitors finish with the same time down to hundredths of a second