| POS | Rank | Heat | BIB | ATHLETE | COUNTRY | RESULTS | ms* | 
|---|---|---|---|---|---|---|---|
| 1 | 1 | 1 | 2113 | Alison DOS SANTOS |  BRA | 48.12 Q | |
| 2 | 2 | 1 | 2331 | Ludvy VAILLANT |  FRA | 48.27 Q | |
| 3 | 1 | 4 | 2362 | Joshua ABUAKU |  GER | 48.32 Q PB | |
| 4 | 1 | 5 | 2971 | Rai BENJAMIN |  USA | 48.35 Q | |
| 5 | 2 | 4 | 2965 | CJ ALLEN |  USA | 48.36 Q | |
| 6 | 3 | 4 | 2537 | Roshawn CLARKE |  JAM | 48.39 Q | |
| 7 | 1 | 2 | 2526 | Kyron MCMASTER |  IVB | 48.47 Q | .464 | 
| 8 | 4 | 4 | 2721 | Ezekiel NATHANIEL |  NGR | 48.47 Q SB | .467 | 
| 9 | 2 | 2 | 2261 | Rasmus MÄGI |  EST | 48.58 Q | |
| 10 | 2 | 5 | 2546 | Jaheel HYDE |  JAM | 48.63 Q | .624 | 
| 11 | 1 | 3 | 2310 | Wilfried HAPPIO |  FRA | 48.63 Q | .628 | 
| 12 | 3 | 5 | 2578 | Kazuki KUROKAWA |  JPN | 48.71 Q SB | |
| 13 | 5 | 4 | 2090 | Julien WATRIN |  BEL | 48.72 q SB | |
| 14 | 3 | 2 | 2968 | Trevor BASSITT |  USA | 48.73 Q | .721 | 
| 15 | 4 | 5 | 2189 | Gerald DRUMMOND |  CRC | 48.73 Q | .722 | 
| 16 | 2 | 3 | 2742 | Karsten WARHOLM |  NOR | 48.76 Q | |
| 17 | 5 | 5 | 2929 | Yasmani COPELLO |  TUR | 48.92 q | |
| 18 | 6 | 5 | 2363 | Emil AGYEKUM |  GER | 49.00 q | |
| 19 | 6 | 4 | 2504 | Mario LAMBRUGHI |  ITA | 49.05 q SB | |
| 20 | 4 | 2 | 2623 | Wiseman Were MUKHOBE |  KEN | 49.10 Q | |
| 21 | 3 | 1 | 2867 | Julien BONVIN |  SUI | 49.19 Q SB | |
| 22 | 4 | 1 | 2171 | Zhiyu XIE |  CHN | 49.25 Q | |
| 23 | 5 | 2 | 2241 | Sergio FERNÁNDEZ |  ESP | 49.26 | |
| 24 | 7 | 4 | 2210 | Vít MÜLLER |  CZE | 49.37 | |
| 25 | 5 | 1 | 2852 | Matic Ian GUČEK |  SLO | 49.40 SB | |
| 26 | 6 | 1 | 2387 | Constantin PREIS |  GER | 49.45 | |
| 27 | 3 | 3 | 2813 | Bassem HEMEIDA |  QAT | 49.50 Q | .495 | 
| 28 | 4 | 3 | 2515 | Alessandro SIBILIO |  ITA | 49.50 Q | .500 | 
| 29 | 7 | 5 | 2061 | Shakeem HALL-SMITH |  BAH | 49.61 | |
| 30 | 5 | 3 | 2868 | Dany BRAND |  SUI | 49.69 | |
| 31 | 6 | 2 | 2932 | İsmail NEZIR |  TUR | 49.92 | |
| 32 | 6 | 3 | 2234 | Pablo Andres IBAÑEZ GUEVARA |  ESA | 50.01 | |
| 33 | 8 | 4 | 2576 | Yusaku KODAMA |  JPN | 50.18 | |
| 34 | 7 | 2 | 2761 | Eric CRAY |  PHI | 50.27 | |
| 35 | 8 | 5 | 2422 | Árpád BÁNÓCZY |  HUN | 50.31 PB | |
| 36 | 7 | 3 | 2465 | Santhosh Kumar TAMILARASAN |  IND | 50.46 | |
| 37 | 9 | 5 | 2849 | Jun Jie Calvin QUEK |  SGP | 50.53 | |
| 38 | 8 | 3 | 2643 | Marc Anthony IBRAHIM |  LBN | 50.62 | |
| 39 | 9 | 3 | 2486 | Malique SMITH |  ISV | 50.86 | |
| 40 | 8 | 2 | 2575 | Takayuki KISHIMOTO |  JPN | 50.90 | |
| 41 | 9 | 2 | 2853 | Andrea ERCOLANI VOLTA |  SMR | 52.69 | |
| 1 | 2560 | Assinie WILSON |  JAM | DNF | |||
| 1 | 2008 | Abdelmalik LAHOULOU |  ALG | DNF | 
ms - Milliseconds - shown when two or more competitors finish with the same time down to hundredths of a second