| POS | Rank | Heat | BIB | ATHLETE | COUNTRY | RESULTS | ms* | 
|---|---|---|---|---|---|---|---|
| 1 | 1 | 5 | 1595 | Bayapo NDORI | 
 BOT                 | 
                44.87 Q PB | |
| 2 | 1 | 1 | 2268 | Wayde VAN NIEKERK | 
 RSA                 | 
                45.18 Q | |
| 3 | 2 | 5 | 1945 | Kirani JAMES | 
 GRN                 | 
                45.29 Q | |
| 4 | 1 | 2 | 2410 | Michael NORMAN | 
 USA                 | 
                45.37 Q | |
| 5 | 2 | 1 | 1566 | Jonathan JONES | 
 BAR                 | 
                45.46 Q | |
| 6 | 1 | 6 | 1874 | Matthew HUDSON-SMITH | 
 GBR                 | 
                45.49 Q | |
| 7 | 1 | 4 | 2351 | Champion ALLISON | 
 USA                 | 
                45.56 Q | |
| 8 | 3 | 5 | 2014 | Nathon ALLEN | 
 JAM                 | 
                45.61 Q | |
| 9 | 3 | 1 | 1872 | Alex HAYDOCK-WILSON | 
 GBR                 | 
                45.62 Q SB | |
| 10 | 2 | 2 | 2041 | Christopher TAYLOR | 
 JAM                 | 
                45.68 Q | |
| 11 | 3 | 2 | 2264 | Zakithi NENE | 
 RSA                 | 
                45.69 Q | |
| 12 | 2 | 4 | 1571 | Dylan BORLÉE | 
 BEL                 | 
                45.70 Q | |
| 13 | 4 | 2 | 1572 | Kévin BORLÉE | 
 BEL                 | 
                45.72 q | |
| 14 | 1 | 3 | 2366 | Michael CHERRY | 
 USA                 | 
                45.81 Q | |
| 15 | 2 | 3 | 2437 | Muzala SAMUKONGA | 
 ZAM                 | 
                45.82 Q | .811 | 
| 16 | 2 | 6 | 2159 | Liemarvin BONEVACIA | 
 NED                 | 
                45.82 Q | .815 | 
| 17 | 3 | 6 | 1736 | Lidio Andres FELIZ | 
 DOM                 | 
                45.87 Q | |
| 18 | 4 | 5 | 2069 | Fuga SATO | 
 JPN                 | 
                45.88 q | |
| 19 | 4 | 1 | 2075 | Julian Jrummi WALSH | 
 JPN                 | 
                45.90 q | |
| 20 | 3 | 4 | 1594 | Isaac MAKWALA | 
 BOT                 | 
                45.93 Q | |
| 21 | 5 | 5 | 1526 | Alex BECK | 
 AUS                 | 
                45.99 q | |
| 22 | 4 | 4 | 2080 | Mikhail LITVIN | 
 KAZ                 | 
                46.00 q SB | |
| 23 | 4 | 6 | 1979 | Christopher O'DONNELL | 
 IRL                 | 
                46.01 q | |
| 24 | 5 | 1 | 1719 | Patrik Å ORM | 
 CZE                 | 
                46.07 | |
| 25 | 3 | 3 | 1579 | Alexander DOOM | 
 BEL                 | 
                46.18 Q | |
| 26 | 5 | 2 | 1731 | Benjamin Lobo VEDEL | 
 DEN                 | 
                46.27 | |
| 27 | 5 | 6 | 2057 | Kaito KAWABATA | 
 JPN                 | 
                46.34 | |
| 28 | 4 | 3 | 2035 | Jevaughn POWELL | 
 JAM                 | 
                46.42 | |
| 29 | 6 | 5 | 2009 | Edoardo SCOTTI | 
 ITA                 | 
                46.46 | |
| 30 | 6 | 2 | 2141 | Luis AVILÉS FERREIRO | 
 MEX                 | 
                46.47 | |
| 31 | 5 | 4 | 2008 | Davide RE | 
 ITA                 | 
                46.49 | |
| 32 | 7 | 2 | 2220 | Kajetan DUSZYŃSKI | 
 POL                 | 
                46.57 | |
| 33 | 6 | 4 | 2321 | Dwight ST. HILLAIRE | 
 TTO                 | 
                46.60 | .596 | 
| 34 | 5 | 3 | 2293 | Ricky PETRUCCIANI | 
 SUI                 | 
                46.60 | .598 | 
| 35 | 7 | 4 | 2282 | Boško KIJANOVIĆ | 
 SRB                 | 
                46.85 | |
| 36 | 6 | 1 | 1551 | Steven SOLOMON | 
 AUS                 | 
                46.87 | |
| 37 | 6 | 3 | 1597 | Anthony PESELA | 
 BOT                 | 
                47.36 | |
| 38 | 6 | 6 | 1605 | Lucas CARVALHO | 
 BRA                 | 
                47.53 | |
| 39 | 7 | 5 | 2154 | Dexter MAYORGA | 
 NCA                 | 
                48.40 | |
| 40 | 7 | 6 | 1502 | Aiden HAZZARD | 
 AIA                 | 
                51.44 SB | |
| 41 | 7 | 3 | 2436 | Obediah TIMBACI | 
 VAN                 | 
                53.32 | 
ms - Milliseconds - shown when two or more competitors finish with the same time down to hundredths of a second