| POS | Rank | Heat | BIB | ATHLETE | COUNTRY | RESULTS | ms* | 
|---|---|---|---|---|---|---|---|
| 1 | 1 | 3 | 1603 | Abderrahman Almoubarrake S ALSALECK |  QAT | 49.08 Q | |
| 2 | 2 | 3 | 1208 | Takatoshi ABE |  JPN | 49.25 Q | |
| 3 | 1 | 1 | 1491 | Karsten WARHOLM |  NOR | 49.27 Q | |
| 4 | 1 | 5 | 691 | Rasmus MÄGI |  EST | 49.34 Q SB | |
| 5 | 2 | 1 | 1066 | Thomas BARR |  IRL | 49.41 Q | |
| 6 | 2 | 5 | 770 | Ludvy VAILLANT |  FRA | 49.49 Q | |
| 7 | 3 | 3 | 1895 | TJ HOLMES |  USA | 49.50 Q | |
| 8 | 3 | 5 | 105 | Abdelmalik LAHOULOU |  ALG | 49.54 Q | |
| 9 | 1 | 2 | 1149 | Kyron MCMASTER |  IVB | 49.60 Q | |
| 10 | 1 | 4 | 1867 | Rai BENJAMIN |  USA | 49.62 Q | .611 | 
| 11 | 3 | 1 | 1045 | Jabir MADARI PALLIYALIL |  IND | 49.62 Q | .619 | 
| 12 | 4 | 1 | 1170 | Kemar MOWATT |  JAM | 49.63 Q | |
| 13 | 2 | 2 | 328 | Alison DOS SANTOS |  BRA | 49.66 Q | |
| 14 | 5 | 1 | 1910 | Amere LATTIN |  USA | 49.72 q | |
| 15 | 4 | 5 | 846 | Chris MCALISTER |  GBR | 49.73 Q | |
| 16 | 5 | 5 | 1772 | Yasmani COPELLO |  TUR | 49.75 q | |
| 17 | 2 | 4 | 1767 | Mohamed TOUATI |  TUN | 49.76 Q | |
| 18 | 3 | 4 | 1527 | Patryk DOBEK |  POL | 49.89 Q | |
| 19 | 6 | 1 | 1400 | Fernando Vega |  MEX | 49.95 q | .949 | 
| 19 | 3 | 2 | 1744 | Chieh CHEN |  TPE | 49.95 Q | .949 | 
| 21 | 4 | 3 | 1452 | Rilwan ALOWONLE |  NGR | 50.04 Q | |
| 22 | 4 | 4 | 585 | Vít MÜLLER |  CZE | 50.15 Q | |
| 23 | 4 | 2 | 904 | Luke CAMPBELL |  GER | 50.20 Q | |
| 24 | 5 | 4 | 1240 | Masaki TOYODA |  JPN | 50.34 q | |
| 25 | 7 | 1 | 1064 | Mahdi PIRJAHAN |  IRI | 50.46 | |
| 26 | 5 | 3 | 1431 | Nick SMIDT |  NED | 50.54 | |
| 27 | 6 | 5 | 1035 | Dharun AYYASAMY |  IND | 50.55 | |
| 28 | 6 | 3 | 1678 | Kariem HUSSEIN |  SUI | 50.62 | |
| 29 | 6 | 4 | 654 | Sergio FERNÁNDEZ |  ESP | 50.71 | |
| 30 | 5 | 2 | 1415 | Creve Armando MACHAVA |  MOZ | 50.76 | |
| 31 | 7 | 5 | 925 | Constantin PREIS |  GER | 50.93 | |
| 32 | 7 | 4 | 336 | Marcio TELES |  BRA | 51.02 | |
| 33 | 7 | 3 | 780 | Wilfried HAPPIO |  FRA | 51.25 | |
| 34 | 8 | 1 | 337 | Artur Terezan |  BRA | 51.52 SB | |
| 35 | 6 | 2 | 1625 | Lindsay HANEKOM |  RSA | 51.71 | |
| 36 | 7 | 2 | 1656 | Ned AZEMIA |  SEY | 52.58 | |
| 37 | 8 | 3 | 1667 | Andrea ERCOLANI VOLTA |  SMR | 52.60 NR | |
| 38 | 8 | 5 | 1081 | Malique SMITH |  ISV | 59.45 | |
| 2 | 389 | Bienvenu Sawadogo |  BUR | DQ | 
ms - Milliseconds - shown when two or more competitors finish with the same time down to hundredths of a second