| POS | Rank | Heat | BIB | ATHLETE | COUNTRY | RESULTS | ms* | 
|---|---|---|---|---|---|---|---|
| 1 | 1 | 3 | 948 | Femke BOL |  NED | 53.90 Q | |
| 2 | 1 | 1 | 1188 | Sydney MCLAUGHLIN-LEVRONE |  USA | 53.95 Q | |
| 3 | 1 | 4 | 1193 | Dalilah MUHAMMAD |  USA | 54.45 Q | |
| 4 | 1 | 2 | 850 | Janieve RUSSELL |  JAM | 54.52 Q | |
| 5 | 1 | 5 | 1218 | Britton WILSON |  USA | 54.54 Q | |
| 6 | 2 | 5 | 810 | Ayomide FOLORUNSO |  ITA | 54.69 Q | |
| 7 | 3 | 5 | 984 | Amalie IUEL |  NOR | 54.70 Q PB | |
| 8 | 2 | 2 | 1183 | Shamier LITTLE |  USA | 54.77 Q | |
| 9 | 2 | 4 | 851 | Shiann SALMON |  JAM | 54.91 Q | |
| 10 | 2 | 1 | 1133 | Anna RYZHYKOVA |  UKR | 54.93 Q | |
| 11 | 3 | 2 | 657 | Viivi LEHIKOINEN |  FIN | 54.95 Q | |
| 12 | 4 | 5 | 834 | Rushell CLAYTON |  JAM | 54.99 Q | |
| 13 | 2 | 3 | 1058 | Zenéy GELDENHUYS |  RSA | 55.05 Q | |
| 14 | 3 | 1 | 614 | Sara GALLEGO |  ESP | 55.09 Q | |
| 15 | 3 | 3 | 999 | Gianna WOODRUFF |  PAN | 55.21 Q | |
| 16 | 4 | 2 | 1136 | Viktoriya TKACHUK |  UKR | 55.27 Q | |
| 17 | 4 | 1 | 451 | Paulien COUCKUYT |  BEL | 55.42 Q | |
| 18 | 4 | 3 | 695 | Jessie KNIGHT |  GBR | 55.48 Q | |
| 19 | 5 | 3 | 823 | Rebecca SARTORI |  ITA | 55.72 q | .716 | 
| 20 | 5 | 2 | 989 | Portia BING |  NZL | 55.72 q | .717 | 
| 21 | 3 | 4 | 412 | Sarah CARLI |  AUS | 55.89 Q | |
| 22 | 5 | 1 | 1080 | Yasmin GIGER |  SUI | 55.90 q SB | |
| 23 | 6 | 2 | 818 | Linda OLIVIERI |  ITA | 56.09 q | |
| 24 | 5 | 5 | 742 | Carolina KRAFZIK |  GER | 56.24 | .231 | 
| 25 | 4 | 4 | 555 | Melissa GONZALEZ |  COL | 56.24 Q | .234 | 
| 26 | 6 | 1 | 1038 | Grace CLAXTON |  PUR | 56.40 | |
| 27 | 6 | 5 | 1046 | Taylon BIELDT |  RSA | 56.67 | |
| 28 | 5 | 4 | 649 | Kristiina HALONEN |  FIN | 56.68 PB | |
| 29 | 7 | 2 | 487 | Aminat JAMAL |  BRN | 56.78 SB | |
| 30 | 7 | 5 | 1027 | Vera BARBOSA |  POR | 56.79 | |
| 31 | 6 | 4 | 542 | Jiadie MO |  CHN | 57.01 | |
| 32 | 7 | 4 | 1070 | Agata ZUPIN |  SLO | 57.12 | |
| 33 | 8 | 4 | 707 | Lina NIELSEN |  GBR | 57.42 | |
| 34 | 8 | 2 | 1106 | Yanique HAYE-SMITH |  TKS | 57.99 | |
| 35 | 7 | 1 | 469 | Chayenne DA SILVA |  BRA | 59.46 | |
| 3 | 1227 | Thi Lan QUACH |  VIE | DQ | |||
| 3 | 1094 | Daniela LEDECKÁ |  SVK | DNF | 
ms - Milliseconds - shown when two or more competitors finish with the same time down to hundredths of a second