| POS | BIB | ATHLETE | COUNTRY | MARK | Reaction Time | 
|---|---|---|---|---|---|
| 1 | 525 | Alexandra Štuková |  SVK | 54.26 Q SB | 0.158 | 
| 2 | 311 | Latoya McDermott |  JAM | 54.35 Q | 0.252 | 
| 3 | 575 | Olha Zemlyak |  UKR | 54.59 Q | 0.157 | 
| 4 | 583 | Meshawn Graham |  USA | 54.96 Q | 0.177 | 
| 5 | 400 | Martina Xuereb |  MLT | 55.24 PB | 0.154 | 
| 6 | 294 | Valentina Zappa |  ITA | 55.57 | 0.169 | 
| 7 | 348 | Bearose Chepkoech |  KEN | 55.83 | 0.152 | 
| 8 | 41 | Sade Sealy |  BAR | 56.14 | 0.219 | 
| POS | BIB | ATHLETE | COUNTRY | MARK | Reaction Time | 
|---|---|---|---|---|---|
| 1 | 559 | Yuliya Baraley |  UKR | 54.96 Q | 0.182 | 
| 2 | 79 | Natalie Geiger |  CAN | 55.00 Q PB | 0.186 | 
| 3 | 277 | Poovamma Raju MACHETTIRA |  IND | 55.15 Q PB | 0.196 | 
| 4 | 399 | Francesca Xuereb |  MLT | 55.57 Q | 0.170 | 
| 5 | 581 | Brianna Frazier |  USA | 55.66 | 0.175 | 
| 6 | 42 | Mara Weekes |  BAR | 56.45 | 0.155 | 
| 7 | 346 | Betty Chelangat |  KEN | 56.71 | 0.127 | 
| 8 | 292 | Chiara Natali |  ITA | 56.71 | 0.144 |