| Pos | Rank | Heat | Bib | Athlete | Mark | Details | Reaction Time | |
|---|---|---|---|---|---|---|---|---|
| 1 | 1 | 1 | 1984 | USA | 54.45  | Q | 0.176 | |
| 2 | 1 | 4 | 1494 | NOR | 54.72 NR | Q | 0.157 | |
| 3 | 1 | 3 | 1989 | USA | 54.87  | Q | 0.194 | |
| 4 | 2 | 1 | 1689 | SUI | 54.98 SB | Q | 0.182 | |
| 5 | 1 | 2 | 1841 | UKR | 55.11  | Q | 0.167 | |
| 6 | 2 | 4 | 373 | BRN | 55.13 PB | Q | 0.221 | |
| 7 | 3 | 4 | 1125 | ITA | 55.20  | Q | 0.229 | |
| 8 | 3 | 1 | 1188 | JAM | 55.20 PB | Q | 0.196 | |
| 9 | 1 | 5 | 1178 | JAM | 55.23  | Q | 0.176 | |
| 10 | 4 | 4 | 2006 | USA | 55.28  | Q | 0.158 | |
| 11 | 5 | 4 | 1433 | NED | 55.32 PB | q | 0.206 | |
| 12 | 2 | 2 | 594 | CZE | 55.33  | Q | 0.160 | |
| 13 | 2 | 3 | 571 | CUB | 55.36  | Q | 0.208 | |
| 14 | 4 | 1 | 131 | ANA | 55.51 SB | Q | 0.251 | |
| 15 | 2 | 5 | 421 | CAN | 55.57  | Q | 0.173 | |
| 16 | 6 | 4 | 255 | BEL | 55.68  | q | 0.159 | |
| 17 | 3 | 2 | 866 | GBR | 55.72 PB | Q | 0.200 | |
| 18 | 4 | 2 | 1141 | ITA | 55.78 SB | Q | 0.211 | |
| 19 | 5 | 2 | 954 | GER | 55.93  | q | 0.185 | |
| 20 | 3 | 3 | 1555 | POL | 55.97  | Q | 0.177 | |
| 21 | 3 | 5 | 856 | GBR | 55.97  | Q | 0.166 | |
| 22 | 5 | 1 | 178 | AUS | 56.00  | q | 0.155 | |
| 23 | 4 | 3 | 1514 | PAN | 56.07  | Q | 0.257 | |
| 24 | 6 | 2 | 1203 | JAM | 56.37  | 0.184 | ||
| 25 | 4 | 5 | 193 | AUS | 56.37  | Q | 0.243 | |
| 26 | 7 | 2 | 541 | COL | 56.49  | 0.161 | ||
| 27 | 5 | 3 | 123 | ANA | 56.79  | 0.170 | ||
| 28 | 5 | 5 | 1138 | ITA | 56.82  | 0.148 | ||
| 29 | 7 | 4 | 202 | AUS | 56.97  | 0.223 | ||
| 30 | 8 | 4 | 1741 | TKS | 56.98  | 0.159 | ||
| 31 | 6 | 5 | 1649 | RSA | 57.11  | 0.182 | ||
| 32 | 6 | 3 | 253 | BEL | 57.15  | 0.138 | ||
| 33 | 7 | 5 | 232 | BAR | 57.37  | 0.177 | ||
| 34 | 6 | 1 | 351 | BRA | 57.66  | 0.189 | ||
| 35 | 8 | 5 | 1385 | MAR | 58.44  | 0.227 | ||
| 36 | 7 | 1 | 1609 | QAT | 1:09.49 PB | 0.230 | ||
| 2 | 1957 | USA | DNF  | 0.181 | ||||
| 3 | 614 | DEN | DQ  | 0.176 | ||||
| 1 | 1506 | NZL | DQ  | 0.178 |